Investigation of Burkholderia cepacia Complex Methylomes via Single-Molecule, Real-Time Sequencing and Mutant Analysis
نویسندگان
چکیده
While the genome sequence determines an organism’s proteins, methylation of nucleotides themselves can confer additional properties. In bacteria, MTs modify specific nucleotide motifs to allow discrimination “self” from “nonself” DNA, e.g., bacteriophages.
منابع مشابه
Burkholderia cepacia complex and cystic fibrosis
The bacteriae previously known as ‘Burkholderia cepacia’ have been reclassified as nine closely related species, sometimes referred to as ‘genomovars’ (Table 1), (Coenye et al, 2001; Mahenthiralingam et al, 2002). Collectively these are commonly referred to as ‘Burkholderia cepacia complex’ (Bcc). The various genomovars have different pathogenic potential (Keig et al, 1999; Bevivino et al, 2002...
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Single-molecule real-time (SMRT) sequencing allows identification of methylated DNA bases and methylation patterns/motifs at the genome level. Using SMRT sequencing, diverse bacterial methylomes including those of Helicobacter pylori, Lactobacillus spp., and Escherichia coli have been determined, and previously unreported DNA methylation motifs have been identified. However, the methylomes of X...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2021
ISSN: ['0021-9193', '1098-5530', '1067-8832']
DOI: https://doi.org/10.1128/jb.00683-20